Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • PurplePancake
    Member
    • Aug 2014
    • 14

    #1

    Determining the biological process or function of genes

    I have a list of DEGs from DESeq from A.digitifera coral. I put them into DAVID software as a list, selected Identifier as "OFFICIAL_GENE_SYMBOL", chose Gene List, and then submitted the list. Then, I chose "Option 1: Convert the gene list to DAVID". None of the genes were in the DAVID database, was what was indicated as the result.

    Is there any software I could use that might be better for my species? I just need to do this (very) quick and dirty (for now). I am interested to determine biological processes or functions of these DEGs I have. Here is a sublist of the DEGs, in case:

    (I appreciate any help!!!!)

    aug_v2a.00154.t1
    aug_v2a.00307.t1
    aug_v2a.00601.t1
    aug_v2a.00603.t1
    aug_v2a.00823.t1
    aug_v2a.00990.t1
    aug_v2a.01279.t1
    aug_v2a.01408.t1
    aug_v2a.01492.t1
    aug_v2a.01496.t1
    aug_v2a.01618.t1
    aug_v2a.02238.t1
    aug_v2a.02239.t1
    aug_v2a.02459.t1
    aug_v2a.03293.t1
    aug_v2a.03318.t1
    aug_v2a.03417.t1
    aug_v2a.03732.t1
    aug_v2a.03955.t1
    aug_v2a.04041.t1
    aug_v2a.04353.t1
    aug_v2a.04482.t1
    aug_v2a.04679.t1
    aug_v2a.04822.t1
    aug_v2a.05042.t1
    aug_v2a.05093.t1
    aug_v2a.05179.t1
    aug_v2a.05185.t1
    aug_v2a.05514.t1
    aug_v2a.05561.t1
    aug_v2a.05667.t1
    aug_v2a.05723.t1
    aug_v2a.05776.t1
    aug_v2a.05823.t1
    aug_v2a.05859.t1
    aug_v2a.05896.t1
    aug_v2a.05945.t1
    aug_v2a.06125.t1
    aug_v2a.06180.t1
    aug_v2a.06372.t1
    aug_v2a.06390.t1
    aug_v2a.06468.t1
    aug_v2a.06469.t1
    aug_v2a.06517.t1
    aug_v2a.06763.t1
    aug_v2a.06991.t1
    aug_v2a.07140.t1
    aug_v2a.07189.t1
    aug_v2a.07227.t1
    aug_v2a.07393.t1
    aug_v2a.07523.t1
    aug_v2a.07683.t1
    aug_v2a.07824.t1
    aug_v2a.08028.t1
    aug_v2a.08251.t1
    aug_v2a.08395.t1
    aug_v2a.08398.t1
    aug_v2a.08439.t1
    aug_v2a.08791.t1
    aug_v2a.08951.t1
    aug_v2a.09028.t1
    aug_v2a.09216.t1
    aug_v2a.09226.t1
    aug_v2a.09253.t1
    aug_v2a.09307.t1
    aug_v2a.09322.t1
    aug_v2a.09376.t1
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    You are working with a very uncommon genome so this is not going to be a trivial task. You are going to have to start blasting the sequences and doing some legwork to assign a putative function to the proteins you have identified as DE.

    You can find the protein sequence for the gene models at the link Devon had posted yesterday: http://marinegenomics.oist.jp/genome...s?project_id=3

    Only 13 proteins from Acropora appear to be in RefSeq: http://www.ncbi.nlm.nih.gov/protein?...from_uid=10529

    Comment

    • dpryan
      Devon Ryan
      • Jul 2011
      • 3478

      #3
      This was also cross-posted on Biostars, where Jeremy Leipzig and I both already replied. Please don't cross-post, it creates double work.

      Comment

      Latest Articles

      Collapse

      • kinpict.com
        Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by kinpict.com
        The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
        Today, 06:37 PM
      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 11-12-2026, 07:59 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      2 responses
      56 views
      0 reactions
      Last Post kinpict.com  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      175 views
      0 reactions
      Last Post SEQadmin2  
      Working...