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  • bio_informatics
    Senior Member
    • Nov 2013
    • 182

    #1

    Download fasta file for pdb id from uniprot

    Hi Members,
    I've a list of pdb id [some ~80].
    I want to download their fasta file from Uniprot.

    I have been unable to find URL which can be then utilized using wget or curl.

    example: 2rh1
    Please help.
    Bioinformaticscally calm
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    If you have 80 perhaps getting the fasta files for UniProt/SwissProt and UniProt/TreEmbl (ftp://ftp.uniprot.org/pub/databases/...base/complete/) then parsing out the sequences you need.

    Comment

    • bio_informatics
      Senior Member
      • Nov 2013
      • 182

      #3
      Thank you GenoM for your reply.

      I downloaded sprot [swissprot] file. On going through it, I found protein name as:

      >sp|Q6GZX3|002L_FRG3G Uncharacterized protein 002L OS=Frog virus 3 (isolate Goorha) GN=FV3-002L PE=4 SV=1

      I have pdb ids with me, and not much information other than that.
      I was wondering how would I be able to get hold my data from this header.

      Appreciate your inputs.

      Originally posted by GenoMax View Post
      If you have 80 perhaps getting the fasta files for UniProt/SwissProt and UniProt/TreEmbl (ftp://ftp.uniprot.org/pub/databases/...base/complete/) then parsing out the sequences you need.
      Bioinformaticscally calm

      Comment

      • bio_informatics
        Senior Member
        • Nov 2013
        • 182

        #4
        I guess 6GZX are the PDB ids. I am randomly grep-ping the ids and have hit few of them.
        But there are multiple entries for one such id.
        Code:
        $ grep -i "2m3g" uniprot_sprot.fasta
        >sp|Q2M3G0|ABCB5_HUMAN ATP-binding cassette sub-family B member 5 OS=Homo sapiens GN=ABCB5 PE=1 SV=3

        >sp|Q2M3G4|SHRM1_HUMAN Protein Shroom1 OS=Homo sapiens GN=SHROOM1 PE=1 SV=1
        Bioinformaticscally calm

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          PDB to Uniprot ID's: http://www.uniprot.org/docs/pdbtosp

          Comment

          • bio_informatics
            Senior Member
            • Nov 2013
            • 182

            #6
            Wow. That helps a lot. Thank you.

            FASTA sequence/file can be downloaded using wget from a URL:
            Ex:
            pdb id -2E9Q
            Code:
            wget "http://www.uniprot.org/uniprot/?query=database%3A%28type%3Apdb+2E9Q%29&format=fasta"
            Note: There can be multiple sequences of one PDB id.

            Last edited by bio_informatics; 09-12-2014, 06:45 AM.
            Bioinformaticscally calm

            Comment

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