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  • heso
    Member
    • May 2014
    • 19

    #1

    UCSC mm10 gtf file

    Hi,
    I have dowloaded UCSC hg19 gtf files from Galaxy



    The resulting file looks like that:
    $ head hg19_genes.gtf
    Code:
    chr1	unknown	exon	11874	12227	.	+	.	gene_id "DDX11L1"; transcript_id "NR_046018_1"; gene_name "DDX11L1"; tss_id "TSS14523";
    chr1	unknown	exon	12613	12721	.	+	.	gene_id "DDX11L1"; transcript_id "NR_046018_1"; gene_name "DDX11L1"; tss_id "TSS14523";
    chr1	unknown	exon	13221	14408	.	+	.	gene_id "DDX11L1"; transcript_id "NR_046018_1"; gene_name "DDX11L1"; tss_id "TSS14523";
    chr1	unknown	exon	14362	14829	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	14970	15038	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	15796	15947	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	16607	16765	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	16858	17055	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	17233	17368	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";
    chr1	unknown	exon	17606	17742	.	-	.	gene_id "WASH7P"; transcript_id "NR_024540"; gene_name "WASH7P"; tss_id "TSS7359";

    When obtaining the same file from UCSC Tables, the file looks somewhat different. First 3 rows are similar and thereafter things don't exactly match.....

    $ head hg19_ucsc_table.gtf
    Code:
    chr1	hg19_knownGene	exon	11874	12227	0.000000	+	.	gene_id "uc001aaa.3"; transcript_id "uc001aaa.3"; 
    chr1	hg19_knownGene	exon	12613	12721	0.000000	+	.	gene_id "uc001aaa.3"; transcript_id "uc001aaa.3"; 
    chr1	hg19_knownGene	exon	13221	14409	0.000000	+	.	gene_id "uc001aaa.3"; transcript_id "uc001aaa.3"; 
    chr1	hg19_knownGene	exon	11874	12227	0.000000	+	.	gene_id "uc010nxr.1"; transcript_id "uc010nxr.1"; 
    chr1	hg19_knownGene	exon	12646	12697	0.000000	+	.	gene_id "uc010nxr.1"; transcript_id "uc010nxr.1"; 
    chr1	hg19_knownGene	exon	13221	14409	0.000000	+	.	gene_id "uc010nxr.1"; transcript_id "uc010nxr.1"; 
    chr1	hg19_knownGene	start_codon	12190	12192	0.000000	+	.	gene_id "uc010nxq.1"; transcript_id "uc010nxq.1"; 
    chr1	hg19_knownGene	CDS	12190	12227	0.000000	+	0	gene_id "uc010nxq.1"; transcript_id "uc010nxq.1"; 
    chr1	hg19_knownGene	exon	11874	12227	0.000000	+	.	gene_id "uc010nxq.1"; transcript_id "uc010nxq.1"; 
    chr1	hg19_knownGene	CDS	12595	12721	0.000000	+	1	gene_id "uc010nxq.1"; transcript_id "uc010nxq.1";

    So, when I grep out miRNA genes from the first gtf file obtained through Galaxy (hg19_genes.gtf ), I get a gtf file with only miRNA genes.
    command:
    Code:
    $cat hg19_genes.gtf | grep "MIR" > hg19_miRNA.gtf
    this command, however, does not work for the file I got from UCSC Tables (hg19_ucsc_table.gtf ). I guess the gene_id's are just denoted to contain miR/MIR symbol.

    So, my question:
    I would like to obtain miRNA gtf files for mm10 in UCSC format (I've used UCSC mm10 for mapping).
    In Galaxy, there is only UCSC mm9 gtf file (https://usegalaxy.org/library_common...9490a8b6c89961).
    How can I obtain UCSC mm10.gtf in a format, where I could grep out the miRNA genes????
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    You can use the table browser at UCSC to export mm10 annotation in GTF format.

    If you happen to have access to iGenomes mm10 download http://support.illumina.com/sequenci...e/igenome.html the GTF file would be in Annotations sub-directory.

    Comment

    • mastal
      Senior Member
      • Mar 2009
      • 666

      #3
      Hi heso,

      The format of the file you downloaded from Galaxy looks like
      the iGenomes files.

      Comment

      • heso
        Member
        • May 2014
        • 19

        #4
        I got it fixed now. I used this site for downloading.
        ftp://ussd-ftp.illumina.com/Mus_musculus/UCSC/mm10/

        There I found the gtf in the format I wanted.
        Thanks everybody

        Comment

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