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  • anibhax
    Member
    • Jan 2013
    • 24

    #1

    Proper regex to mark duplicates using Picard tools on SOLiD data

    Hi,

    I'm having trouble removing duplicates using Picard tools on SOLiD data. I get a regex not matching error.

    The reads have the following names:

    Code:
    22_758_632_F3
    
    604_1497_576
    
    124_1189_1519_F5
    
    358_1875_702_F5-DNA
    And I don't think Picard tools is able to pick these read names with its default regex.

    I tried to change the default regex. This time it does not throw an error, but it takes too long and times out (out of memory). I suspect I'm not giving the right regex. Here is my command:

    Code:
    java -jar $PICARD_TOOLS_HOME/MarkDuplicates.jar I=$FILE O=$BAMs/MarkDuplicates/$SAMPLE.MD.bam M=$BAMs/MarkDuplicates/$SAMPLE.metrics READ_NAME_REGEX="([0-9]+)_([0-9]+)_([0-9]+).*"
    Any help is appreciated. Thanks!

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