how to estimate a specific gene expression which is not in the DE list

[shirley47162928]

Hi,
I want to see some specific genes expression between 2 groups. But these genes are not significantly different expressed after running edgeR.
What should I do?

[Gordon Smyth]

You can get a topTags table for all genes, then look at the ones you are interested in:

tab <- topTags(mydata, n=Inf)
i <- tab$GeneID %in% MyGenes
tab[i,]

[bioBob]

Plot the counts by replicate. Normalized.