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  • apratap
    Member
    • Jan 2009
    • 58

    Lateral Gene Transfer Studies using PE data

    Hi Guys

    I am wondering if someone here has done or has some experience studying gene transfers between two species using GA PE data.

    We have two species who we know coexist. We have done sequencing and have good PE data. I am trying to figure out if there are any signs of gene transfer using PE data through singeltons.

    If you have any interest or experience, I will be very happy to talk to you.

    Best,
    -Abhi

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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