Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • popo
    Junior Member
    • Feb 2015
    • 1

    #1

    consensus sequence with samtools and bcftools 1.2

    Hi,

    I want to build a consensus sequence for a small region of the genome from an individual in 1000genomes. Here's what I did:

    Code:
    samtools view -b ftp://ftp-trace.ncbi.nih.gov/1000genomes/ftp/data/HG00096/alignment/HG00096.mapped.ILLUMINA.bwa.GBR.low_coverage.20120522.bam 17:7512445-7512545 -o HG00096_1.bam
    Code:
    samtools mpileup -uf ../../1kg/human_g1k_v37.fasta HG00096_1.bam | bcftools view - -o HG00096_1_w_rg.vcf
    #output
    [mpileup] 1 samples in 1 input files
    <mpileup> Set max per-file depth to 8000


    Code:
    vcfutils.pl vcf2fq HG00096_1_w_rg.vcf
    #error output
    substr outside of string at /usr/local/bin/vcfutils.pl line 557, <> line 393.
    Use of uninitialized value in lc at /usr/local/bin/vcfutils.pl line 557, <> line 393.
    substr outside of string at /usr/local/bin/vcfutils.pl line 557, <> line 393.


    So I had a look at the vcf file, and found that values for the alt column were always <X>, which vcfutils treated as indels. The line of code generated the errors, line 557, looked like:
    Code:
    substr($$seq, $beg, $end - $beg) = lc(substr($$seq, $beg, $end - $beg));
    As all rows in the vcf file were treated as indels, the length of $seq didn't increase while $beg took the values of the starting position of all input lines.

    Is this a bug, or it's the way vcfutils designed to behave? Is there any fix to this? It's quite possible that I was missing something very basic because I'm new to this field. Any suggestion would be helpful.

    Thanks.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 10:52 AM
0 responses
9 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-13-2026, 09:56 AM
0 responses
16 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-09-2026, 11:14 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Working...