Raw 16S Metagenomic data
I have dual indices paired end sequences in separate fastq.gz files.
Using EDAMAME's tutorial of using pandaseq I was able to join the paired end. However I do not understand how to do the Automated paired-end merging? Do I make the list as a txt file and save it in the same document? The shell script returns the error that the file in not found even if I drag and drop location of the file.
Also, how do I extract the indices out?
And, for the mapping file, Under Barcode Column, Do i combine both the indice? Under sample ID will it be separate for the forward and reverse strand?
If there is A QIIME for dummies I would greatly appreciate it! :P
Using EDAMAME's tutorial of using pandaseq I was able to join the paired end. However I do not understand how to do the Automated paired-end merging? Do I make the list as a txt file and save it in the same document? The shell script returns the error that the file in not found even if I drag and drop location of the file.
Also, how do I extract the indices out?
And, for the mapping file, Under Barcode Column, Do i combine both the indice? Under sample ID will it be separate for the forward and reverse strand?
If there is A QIIME for dummies I would greatly appreciate it! :P
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