Hi,
Possibly a very dumb question, but if I have a dataset of paired-end, 150bp reads, but a computational tool that is formatted to analyze single-end 50bp reads. I am wondering if it is practically feasible to only consider 1 end for each read, and clip that read to 50 bp, and use it. Also, is it logical to do so, in other words am I missing some reason why this is a bad idea?
Thanks for any help!
Possibly a very dumb question, but if I have a dataset of paired-end, 150bp reads, but a computational tool that is formatted to analyze single-end 50bp reads. I am wondering if it is practically feasible to only consider 1 end for each read, and clip that read to 50 bp, and use it. Also, is it logical to do so, in other words am I missing some reason why this is a bad idea?
Thanks for any help!
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