I am attempting to do a local blast nt. I have implemented the following procedure:
The command I run is as follows:
Is the output given. At first, I thought this was because it could not read my the location of my local db. So I made sure I had appropriately extracted the tarball. I have.
Is there something wrong with my .fasta file? All I want to do is blast the local nt db against it and see what hits/matches I get. So I am quite confused as to why I am getting an error.
- update_blast.pl nt
- tar zvpf nt.01.tar.gz command for each volume downloaded
- ran a simple bash command (which fails)
The command I run is as follows:
Code:
blastn -query ~/programming/blastexamples/example2/db/even_tribolium.fasta -db ~/localblastdb -task blastn -html -out out.html
blastn -query ~/programming/blastexamples/example2/db/even_tribolium.fasta -db ~/localblastdb -task blastn -html -out out.html
BLAST Database error: No alias or index file found for nucleotide database [/home/heath/localblastdb] in search path [/home/heath/programming/blastexamples/example2::]
BLAST Database error: No alias or index file found for nucleotide database [/home/heath/localblastdb] in search path [/home/heath/programming/blastexamples/example2::]
Is there something wrong with my .fasta file? All I want to do is blast the local nt db against it and see what hits/matches I get. So I am quite confused as to why I am getting an error.
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