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In RNA-Seq, While we have our list of DE genes, selecting a specific transcript/isoform for gene synthesis is not straight forward. How to generate a transcript sequence derived from a contig of the transcript sequences obtained in the RNAseq experiments? Cheers
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Are you asking if an overall consensus sequence can be generated for a gene from a set of mapped reads?Last edited by GenoMax; 03-10-2015, 09:11 AM. -
If you have a linear contig then isn't that the consensus sequence for that transcript or are you referring to a region in the alignment file (where multiple reads are piled up) that you want to derive a consensus sequence from?Comment
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For a particular gene, what is the splice variance ? from Alignment file e.g. bam or sam.Originally posted by GenoMax View PostComment
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Originally posted by GenoMax View Post
‘If you have a linear contig then isn't that the consensus sequence for that transcript’
Yes. And how to get the contigs for each?
I want output a sequence as a result of generating a contig from the mapped sequence data. Thank you!Last edited by super0925; 05-29-2015, 02:44 AM.Comment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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