In RNA-Seq, While we have our list of DE genes, selecting a specific transcript/isoform for gene synthesis is not straight forward. How to generate a transcript sequence derived from a contig of the transcript sequences obtained in the RNAseq experiments? Cheers
-
-
Are you asking if an overall consensus sequence can be generated for a gene from a set of mapped reads?Last edited by GenoMax; 03-10-2015, 09:11 AM. -
If you have a linear contig then isn't that the consensus sequence for that transcript or are you referring to a region in the alignment file (where multiple reads are piled up) that you want to derive a consensus sequence from?Comment
-
For a particular gene, what is the splice variance ? from Alignment file e.g. bam or sam.Originally posted by GenoMax View PostComment
-
Originally posted by GenoMax View Post
‘If you have a linear contig then isn't that the consensus sequence for that transcript’
Yes. And how to get the contigs for each?
I want output a sequence as a result of generating a contig from the mapped sequence data. Thank you!Last edited by super0925; 05-29-2015, 02:44 AM.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment