Hi All,
I'm having Illumina, paired end reads for a bacterial genome. I'm newbie in NGS, paired end, etc terms.
I ran fastqc on forward and reverse reads.
My question:
In my output, report [basic statistics plot] shows poor quality for R2 than R1. Is there any bias while sequencing?
I know I'm putting a broad question and may sound lame.
I'm having Illumina, paired end reads for a bacterial genome. I'm newbie in NGS, paired end, etc terms.
I ran fastqc on forward and reverse reads.
My question:
Why are reports different for R1 and R2?
I know I'm putting a broad question and may sound lame.
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