Troubles with mrsFAST

[ClemBuntu]

Hello everybody,

I'm trying to use mrsFast however I get this error :

ERR: Inconsistent read length for @NS500354:3:H052NAFXX:1:11101:21889:1027

Of course my reads do not have all the same size because I trimmed it :

grep -A 1 "@NS500354:3:H052NAFXX:1:11101:21889:1027" *fastq
allReads_R1.fastq:@NS500354:3:H052NAFXX:1:11101:21889:1027 1:N:0:TAAGGC
allReads_R1.fastq-NTACANCACTGTGTATGAAACTATGTGCTTAACTTCATGAAATCCTACT
--
allReads_R2.fastq:@NS500354:3:H052NAFXX:1:11101:21889:1027 2:N:0:TAAGGC
allReads_R2.fastq-NNNNAGGATTTCNNNANGTTAAGCACATAGNNNCATACACNGTGTTGTATCTGNNTCTNNNACACATCTGACGCNNNCGNNNNTCTTACGCGTGTAGANNNNNGNGNTCGCCGTATCNTNNANAANAAANGGGGGGGGGGGGGNGNNNNG

Does this error mean all my reads have to have the same length or am I doing something wrong ?

Thanks

[GenoMax]

mrsFAST manual indicates that a decision on the window size needs to be made based on the length of the reads. Perhaps for this reason it expects the reads to be of uniform length.