Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • skblazer
    Member
    • Feb 2009
    • 51

    Can BWA automatically identify the quality format?

    I have several sets of raw Solexa reads in fastq file.

    But the quality of reads in some files are in the Illumina format,
    just like
    eecceffdeeffdfeffdfdcbaaB`b]bbcc\ceaaa_a`c^`ad^\c`Lb\

    some in the standard Phred format.
    just like
    TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTPQPQQNNNNNNNNN

    I want to know whether the current alignment tools can identify the quality format automatically?

    Thank you.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    I think MIRA will guess automatically (and you can tell it explicitly), but believe it would still expect them to be consistent.

    Can you tell from the filenames or read names, and convert them to a consistent state?

    Comment

    Latest Articles

    Collapse

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 10:09 AM
    0 responses
    10 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-02-2026, 12:03 PM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-02-2026, 11:40 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Working...