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  • skblazer
    Member
    • Feb 2009
    • 51

    #1

    Can BWA automatically identify the quality format?

    I have several sets of raw Solexa reads in fastq file.

    But the quality of reads in some files are in the Illumina format,
    just like
    eecceffdeeffdfeffdfdcbaaB`b]bbcc\ceaaa_a`c^`ad^\c`Lb\

    some in the standard Phred format.
    just like
    TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTPQPQQNNNNNNNNN

    I want to know whether the current alignment tools can identify the quality format automatically?

    Thank you.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    I think MIRA will guess automatically (and you can tell it explicitly), but believe it would still expect them to be consistent.

    Can you tell from the filenames or read names, and convert them to a consistent state?

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