I learned that estimated false positive SNP rate=1- (observed_ti/tv_ratio-0.5)/(expected_ti/tv_ratio-0.5). In one of our re-sequencing data, the observed ti/tv ratio for coding region is 3.0 (while expected ti/tv ratio for exome is 2.8). But false positive rate can not be negative...
-
-
What do ti and tv stand for? -
-
Does anybody know why the false positive SNP rate is estimated as
1- (observed_ti/tv_ratio-0.5)/(expected_ti/tv_ratio -0.5)?
Any reference to this is much appreciated.Comment
-
-
Thanks Nilshomer! I will have a look at the website you pointed at, and hope I can figure out the answer to my question...Originally posted by nilshomer View PostComment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment