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RSEM expected counts and technical replictaes

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  • RSEM expected counts and technical replictaes

    Hi all,

    does any one know if it is correct to sum the calculated expected_count from technical replicates (different lane runs) calculated in RSEM by running rsem-calculate-expression separately on them?.

    Many thanks in advance,
    Raul2

  • #2
    I would be rather surprised if that yielded the same values as if you had merged beforehand. Granted, I expect the difference won't be that huge, but they will likely be different.

    Comment


    • #3
      Thank you!

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