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  • 3000 paired end library titration run

    Hello
    I have reads from 3000 Paired end library of 454. with this there is also a folder named titration run containing files of ~500MB. Can you pls tell me whether I should include this data during assembly? If yes then what are the parameters to include this? I am a real newbie in next generation sequencing. Your help will be great
    Thanks
    Sabrina

  • #2
    If you are sure the titration data is from the same library, and the run was successful, you definitively can include it. Titration runs are done to optimize the DNA molecule/bead ratio, and should yield good reads...

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