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3000 paired end library titration run

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  • flxlex
    replied
    If you are sure the titration data is from the same library, and the run was successful, you definitively can include it. Titration runs are done to optimize the DNA molecule/bead ratio, and should yield good reads...

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  • sabrinaelias
    started a topic 3000 paired end library titration run

    3000 paired end library titration run

    Hello
    I have reads from 3000 Paired end library of 454. with this there is also a folder named titration run containing files of ~500MB. Can you pls tell me whether I should include this data during assembly? If yes then what are the parameters to include this? I am a real newbie in next generation sequencing. Your help will be great
    Thanks
    Sabrina
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