HI,
I have RNAseq data from samples which has to be aligned to reference Transcriptome. Later I would like to compute number of times a particular nucleotide in reference transcript has been mutated to another nucleotide. For an instance number of times nucleotide "A" in reference transcript is mutated to "C" in treated_transcript.
So far I used RNAseq for quantifying gene expression and finding the differential gene expression.
Any guidance would be highly appreciated.
Thanks in advance.
I have RNAseq data from samples which has to be aligned to reference Transcriptome. Later I would like to compute number of times a particular nucleotide in reference transcript has been mutated to another nucleotide. For an instance number of times nucleotide "A" in reference transcript is mutated to "C" in treated_transcript.
So far I used RNAseq for quantifying gene expression and finding the differential gene expression.
Any guidance would be highly appreciated.
Thanks in advance.
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