Where can I find sequence reads?

[Saeideh]

Hi~~

Where can I download sequence reads directly? (without installing applications; like ftp)

I want small cDNA reads to test TopHat and cufflinks fast. I tried to design a file with a few reads but because there is bias, the quality of my reads are so low.

Thank you..

[westerman]

Most web browsers offer direct FTP support via:

ftp://username[email protected]

I realize that you are in Iran and thus may have a more limited web browser but I suggest trying out your web browser first before writing off FTP

As for where to get the reads, I suggest the SRA at NCBI but it does depend on what organism you want. NCBI does like to use Aspera as a download method but you should be able to step around that.

[GenoMax]

@Saeideh: If you are unable to use the download methods then try "randomreads.sh" program from BBMap suite. You can provide it a fasta reference to generate random reads with characteristics you can control: http://seqanswers.com/forums/showthread.php?t=48988