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  • ticirocha
    Junior Member
    • Sep 2015
    • 2
    #1

    Evaluating RAST annotation

    Hi everyone,
    I'm doing an annotation using RAST in a non-ordered contigs genome and I'm having trouble to determine if the annotation was done properly or not, because the reference genomes avaible are not that close to my microorganism... so I have almost half of my CDS not agrouped in subsystems, and also, I can't tell how bad it is.
    How can I evaluate the quality of the annotation? How can I decide if a CD is properly annotated or not?
    Can anyone help me, please ?
    Tags: contigs, genome annotation, rast
    • cliffbeall
      Senior Member
      • Jan 2010
      • 144
      #2
      I did something like this recently and about 40% of genes did not get a COG assignment. One way to evaluate the quality would be to check for a list of core housekeeping genes to make sure they are present and correctly annotated.

        Comment

        • ticirocha
          Junior Member
          • Sep 2015
          • 2
          #3
          Thank you Cliffbeall! That's what I did... the ones that interests me are there and well annotated... I think I'll move on like that...
          thanks again!

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