Hi all,
I am working on a piece of data of a kind I've never dealt with before, and I'd like your input if anybody has experience dealing with things like this. I have an Agilent two-color microarray with 8 sub-arrays (1_1.. 1_4, 2_1..2_4) that involve 3 conditions (Control, Delta, NS). Biological replicas were combined into one pool each (don't ask), so there's basically only technical variation to assess.
If you can help me with suggestions how can I analyze it I would appreciate it. Here's is the design scheme:
I am working on a piece of data of a kind I've never dealt with before, and I'd like your input if anybody has experience dealing with things like this. I have an Agilent two-color microarray with 8 sub-arrays (1_1.. 1_4, 2_1..2_4) that involve 3 conditions (Control, Delta, NS). Biological replicas were combined into one pool each (don't ask), so there's basically only technical variation to assess.
If you can help me with suggestions how can I analyze it I would appreciate it. Here's is the design scheme:
