I have a collapsed fasta files with all my counts and the corresponding sequences. I would like to annotate those sequences onto a gff file to find out what they are. Is this possible? Usually I would need a bam/sam file, but I dont have that for these sequences.
-
-
So map the collapsed fasta file to something and then count accordingly. Just make the counts part of the read name so you can get them back later. BTW, you made your life more difficult by collapsing things like that. Aligners are fast enough these days to handle whatever size data you want to throw at them.
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment