How to tell legacy_blast.pl to look for blastp in different path?

[incubusfang]

Hello,
I was testing pantherScore.pl but then I got this :

Can't exec "/usr/bin/blastp": No such file or directory at /tmp_home/lf/sf/ncbi-blast-2.2.30+/bin//legacy_blast.pl line 85.

It seemed that pantherScore.pl initiated a blastp process but the legacy_blast.pl couldn't find blastp.

By typing "which blastp", I got /tmp_home/lf/sf/ncbi-blast-2.2.30+/bin/blastp

And then I thought I could just modify that line 85 yet there was no room for me to modify it (http://www.ncbi.nlm.nih.gov/IEB/Tool...egacy_blast.pl)

Can anyone tell me how to solve this? Many thanks

[WhatsOEver]

I assume that your blastp is simply not found in any dir in the path, not specifically in "/usr/bin"?! The easiest way to resolve the error would then be to create a symlink in /usr/bin pointing to your blastp installation.

sudo ln -s /tmp_home/lf/sf/ncbi-blast-2.2.30+/bin/blastp /usr/bin/blastp

[maubp]

It looks like you are expected to edit this line near the start of legacy_blast.pl:

# Default PATH where binaries will be found
use constant DEFAULT_PATH => "/usr/bin";

In your case, I would try:

# Default PATH where binaries will be found
use constant DEFAULT_PATH => "/tmp_home/lf/sf/ncbi-blast-2.2.30+/bin";