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Sorry, I thought biostars and seqanswers were independent forums.Comment
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SeqAnswers and Biostars are indeed independent and you are welcome to cross-post on SeqAnswers. We generally provide a link (biostars) for cross-posted questions (if the original post does not provide one, like @Brian did) so people can check if the question has been answered on biostars before composing (a lengthy) reply here.
That said, some moderators on Biostars don't like questions being cross-posted to other forums but that appears to be biostar specific policy.Last edited by GenoMax; 02-28-2016, 10:56 AM.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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