Hi, i have chip-seq data from an isogenic cell model where i am looking to determine which peaks overlap between various data-sets and also what the changes in fold enrichment are between them. I have considered using bedtools intersect to determine overlaping peaks but how can i determine the difference in fold enrichment, many peaks share location but differ in enrichment. This is for TF chip.
-
The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...Today, 05:41 AM -
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment