Hi everybody!!!
I am using the Unified Release of PbShort, to analyze some 454 sequences, but I have a couple of questions. I would like to know how to convert an alignment file to .bam. I mean I try to do it with MosaikText, and the -bam parameter, but it didn't work, ant it says that this parameter is not recognized, and at the Alignment archive options -bam parameter does not appear.
And by the other side, using gigaBuild when I run it it says ERROR: inconsistent unpadded read lengths for read=GLMZ0L003F9IDP ace=217 fasta=227. terminating... , but anyway the output file was created, so do you thing the file is ok? and just don't take in count that read?
Thanks,
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...