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I am doing some QC on my illumina sequencing raw data. One of the test I saw other people ran is to do a nucleotide distribution calculation. Is this really some useful information? How do I use it.
Thanks
Thanks
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Perhaps download the FastQC package to explore your read statistics with a simpel GUI: http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/ -
Yes the nucleotide distribution is useful.
If the distribution seems "uneven" at the beginning, that could indicate some residual adaptor sequences. The unevenness should be obvious when you compute the distributions of each base.Comment
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