I am doing some QC on my illumina sequencing raw data. One of the test I saw other people ran is to do a nucleotide distribution calculation. Is this really some useful information? How do I use it.
Thanks
Thanks
-
-
Perhaps download the FastQC package to explore your read statistics with a simpel GUI: http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/ -
Yes the nucleotide distribution is useful.
If the distribution seems "uneven" at the beginning, that could indicate some residual adaptor sequences. The unevenness should be obvious when you compute the distributions of each base.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment