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Hi, I would like to know if it is possible to simulate a small RNA-seq dataset because I found tools only for RNA seq.
Thank you.
Thank you.
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Perhaps you could clarify your goal? -
I would like to have a synthetic small RNA-seq for validation purposes.Comment
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That's pretty vague. Since you are not stating any specific goals or metrics, I recommend you download some real small-RNA data from somewhere (such as SRA) and use that for validation. If you are not trying to measure anything in particular, real data is always better than synthetic data.Last edited by Brian Bushnell; 07-13-2016, 07:23 PM.Comment
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Are you looking for small (as in amount of reads/data) or small as in small RNA-seq data?Comment
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Small as in small RNA-seq data, like a real small rna sequencing for miRNAs.Comment
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Thank you.Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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