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  • RikyJKD
    Member
    • Jul 2014
    • 17

    #1

    Simulation of a small RNA-seq experiment

    Hi, I would like to know if it is possible to simulate a small RNA-seq dataset because I found tools only for RNA seq.
    Thank you.
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    Perhaps you could clarify your goal?

    Comment

    • RikyJKD
      Member
      • Jul 2014
      • 17

      #3
      I would like to have a synthetic small RNA-seq for validation purposes.

      Comment

      • Brian Bushnell
        Super Moderator
        • Jan 2014
        • 2709

        #4
        That's pretty vague. Since you are not stating any specific goals or metrics, I recommend you download some real small-RNA data from somewhere (such as SRA) and use that for validation. If you are not trying to measure anything in particular, real data is always better than synthetic data.
        Last edited by Brian Bushnell; 07-13-2016, 07:23 PM.

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          Are you looking for small (as in amount of reads/data) or small as in small RNA-seq data?

          Comment

          • RikyJKD
            Member
            • Jul 2014
            • 17

            #6
            Small as in small RNA-seq data, like a real small rna sequencing for miRNAs.

            Comment

            • GenoMax
              Senior Member
              • Feb 2008
              • 7142

              #7
              I don't know of a simulation program that can generate smallRNA seq data. Can you use a real dataset from here?
              Last edited by GenoMax; 07-14-2016, 05:26 AM.

              Comment

              • RikyJKD
                Member
                • Jul 2014
                • 17

                #8
                Thank you.

                Comment

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