Novoalign error message:- Interrupted..11 Obtained 7 stack frames.

[sandhya]

Hi,

I receive the following error while aligning paired-end reads using novoalign.

Interrupted..11
Obtained 7 stack frames.
0x400348
0x4003cd
0x43d860
0x40df50
0x400d07
0x4d03f6
0x400239

The command I ran was ./novoalign -F STDFQ -r0.2 -d HIV1.fas -f read1.fq read2.fq > paired.novoalign

If this is an OS/CPU related problem with a fix or if there is anything else I need to check please advise.

P.S - I have posted the same query in Novocraft's forum but since there has not been a reply I decided to post it here as well. Sorry for cross-posting.

[sandhya]

Posting the reply from Novocraft's forum for this thread from zayed
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On Tue 14 of Sep., 2010 12:48 MYT, by zayed
OK, it looks as though you may not have built your database index before doing the alignment step.

First ensure that you index your fasta database sequence(s) using novoindex:

./novoindex HIV1.nix HIV1.fas



Now do the alignment as before

./novoalign -F STDFQ -r0.2 -d HIV1.nix -f read1.fq read2.fq > paired.novoalign
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And it worked