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  • percy
    Junior Member
    • May 2009
    • 2

    #1

    problem of viewing assembly data in Tablet

    Dear all:
    I want to use Tablet to view assembly data (afg file ) from velvet, but it show error messages when I import the data. Can someone tell me what is mean:
    “ an error has occurred while attempting to open the provided assembly.
    Tablet.io.ReadException:Tablet encountered a problem on line 200,968.258 of “velvet_asm.afg”.
    Error details:java.lang.NumberFormatException: Value out of range. Value:”65485” Radix:10 “.

    Thanks
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    If one of the Tablet authors doesn't spot this post, email [email protected] for help.

    Comment

    • GStephen
      Junior Member
      • Mar 2010
      • 9

      #3
      Hi, I get the feeling (but can't be sure) that this is a malformed AFG file; possibly the quality scores given the error. If you could either strip out the relevant line from the file, or send me the whole file I could at least confirm this and see if there's anything that could be done about it.

      Comment

      • percy
        Junior Member
        • May 2009
        • 2

        #4
        thanks a lot.
        the file is too large, so I strip some lines and post here.
        200,968,248 src:10943011
        200,968,249 off:-4
        200,968,250 clr:90,0
        200,968,251 }
        200,968,252 {TLE
        200,968,253 src:11541127
        200,968,254 off:-3
        200,968,255 clr:90,0
        200,968,256 }
        200,968,257 }
        200,968,258 {CTG
        200,968,259 iid:54
        200,968,260 eid:60-1
        200,968,261 seq:
        200,968,262 AGACTGTTTATATTAGGATTGTCAAGACACTCCGGTATTACTCGAGCCCGTAATACAACA
        200,968,263 GAGCGTCCTTAATAAATATTTCTATAAATATTTGATGTTTACCTTCCGAAAAAGGTACAT
        200,968,264 TGCCAACCTTATTTGATAAATGTGTAAACTAAGATAAGTGTTGAGATCGGATTTTGATAA
        200,968,265 AGGCATTTGTTTGGGGTGTATGAGTTGGGAAAAGAAACAACAATCATCTCTTGACCAGTT
        200,968,266 TTCCTACTACTAGTGTGTTATCATATAGCGTAAGAGTATGACATAGAGATTGAGAAACAG
        200,968,267 AAACTGAATCATAACGAACATCACAGAATTTACATTTCTACACAATTTTCAGAAAAAGTG
        200,968,268 GAGTGGTGATATATGCTTATTGTTCTACAAGTTCTCTTCATGCGTTTTTGTTTATCTATC

        Comment

        • GStephen
          Junior Member
          • Mar 2010
          • 9

          #5
          Thanks,

          I'll look into this and get back to you.

          Comment

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