Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • vivi7
    Member
    • Mar 2014
    • 10

    #1

    Unknown error with Stampy

    Hi all,

    I am trying to do some paired-end mapping to a genome with Stampy, and I get an error that I don't manage to find any information about:


    [b085841@stacks_vs02 stampy-1.0.23]$ python2.7 ./stampy.py -g Genome -h HT --substitutionrate=0.15 -t 19 -M ./B_Clean.merged.1.fq ./B_Clean.merged.2.fq > ./B_Mapped_09.08

    ​Stampy: Mapping...

    stampy: Error: [Errno 121] Remote I/O error​

    I was running several analyses and they crashed after more than a week that they were running, if I try to restart the runs they crash almost immediately now.
    I have tried to search for similar issues online and here on SEQanswers but I don't seem to find any information about what is the problem.

    Have you ever seen such problem before? Any input, if possible, will be greatly appreciated.

    Thanks in advance
    Virginia
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Are the files hosted on a different server? The error suggests that it's a file sharing issue and has nothing to do with stampy.

    Comment

    • vivi7
      Member
      • Mar 2014
      • 10

      #3
      Thanks!

      Originally posted by dpryan View Post
      Are the files hosted on a different server? The error suggests that it's a file sharing issue and has nothing to do with stampy.
      Thank you very much!
      It turned out that several servers shared an area on a filer that wasn't working... so now we can solve the problem (hopefully)!
      Thanks super much for the help!!!!

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 07:59 AM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      32 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      0 responses
      45 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      171 views
      0 reactions
      Last Post SEQadmin2  
      Working...