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Maybe a simple question, but what is your preferred style for listing DNA length in a publication. Do you use b.p. for double stranded DNA and nt for single stranded DNA or RNA? Then there is the kb vs. knt possibility (if ever?) Is there anywhere where preferred style is found, do some journals have a preference?
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bp/kb is what I have generally seen used. -
Oligo makers describe their ssDNA oligos as 20 bases or nucleotides. I wouldn't use bp for ssDNA. It does raise some fine hair-splitting questions: if you are cloning by restriction digestion, how do you describe the resulting fragment that is dsDNA plus ssDNA overhangs? Most people would describe it as the length between the cut sites, like 374 bp, even though only 370 nucleotides are base paired.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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