Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • JamesSeward
    Member
    • Jul 2016
    • 11

    usearch memory problem

    Hello all,

    I am currently trying to run derep_fulllength with my combined_seqs.fna file, and I'm getting this memory error:

    derepfull.cpp(34): Out of memory, mymalloc(9973772, 4), curr 8.9e+08 bytes, total 9.29e+08 (929Mb)

    would anyone know a way around this besides buying the 64 bit version?

    Thanks in advance for any help.

    James
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Are you deduplicating a set of sequences?

    There is also vsearch which is a free option for 64-bit instead of usearch.

    Comment

    • JamesSeward
      Member
      • Jul 2016
      • 11

      #3
      Originally posted by GenoMax View Post
      Are you deduplicating a set of sequences?

      There is also vsearch which is a free option for 64-bit instead of usearch.
      GenoMax,

      Thank you very much, I just tried it with vsearch and that seemed to have solved my problem!

      Thank you again,

      James

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM
      • SEQadmin2
        From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
        by SEQadmin2


        Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


        The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
        ...
        06-02-2026, 10:05 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      12 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      48 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-09-2026, 11:58 AM
      0 responses
      106 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-05-2026, 10:09 AM
      0 responses
      125 views
      0 reactions
      Last Post SEQadmin2  
      Working...