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MeFit - does it work?
Hello, I am new to NGS analysis and am considering using MeFit to merge and filter my 16s rRNA data from MiSeq. I requested PE300 (now I regret it) for V3-4 and the reverse read is poor. This program will allow me to keep the sequences that don't actually overlap in the analysis if they are good enough
. It sounds really great and will let me use mothur to complete the analysis, I am currently using mothur from step one. I just don't know if it is really going to work to improve my data and I don't understand enough to really assess this based on what the paper says so I'm asking for help to figure this out.
Here is a link to the paper: http://bmcbioinformatics.biomedcentr...859-016-1358-1
Any input from people who have done this before (analysis with NGS or using MeFit) would be greatly appreciated!
. It sounds really great and will let me use mothur to complete the analysis, I am currently using mothur from step one. I just don't know if it is really going to work to improve my data and I don't understand enough to really assess this based on what the paper says so I'm asking for help to figure this out.Here is a link to the paper: http://bmcbioinformatics.biomedcentr...859-016-1358-1
Any input from people who have done this before (analysis with NGS or using MeFit) would be greatly appreciated!