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When using prinseq on paired end data and I want to trim the 5' end of both reads by 10 bases, how can I do it?
I've tried -trim_left 10 because I assumed it would do the same for both. Maybe thats wrong and it would trim from 5' of _1 and 3' of '2?
At any rate it doesn't seem to trim them at all for either file when I run the filtered reads through fastqc. Anyone ever try this or know how to do it correctly? Thanks!
I've tried -trim_left 10 because I assumed it would do the same for both. Maybe thats wrong and it would trim from 5' of _1 and 3' of '2?
At any rate it doesn't seem to trim them at all for either file when I run the filtered reads through fastqc. Anyone ever try this or know how to do it correctly? Thanks!
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