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  • Paired end solexa (goat/gerald) pipeline

    Hi,

    I am unable to go beyond the Solexa pipeline on paired end data. The data is 72 cycles of images in a single folder (36 x 2). I am not really sure of what the config.txt file should be!
    Any ideas?

    $ cat config.txt
    ANALYSIS sequence_pair
    USE_BASES Y35n,Y35n
    SEQUENCE_FORMAT --fastq
    ELAND_MULTIPLE_INSTANCES 6

    This one says:
    Found 1 reads.
    Read 1: length 72.
    Overriding lengths using option 72.
    Error: Too many reads specified in USE_BASES=Y35n,Y35n.
    Removing temporary file /tmp/tmp-GERALD-4440-4807.txt
    Error: ../Gerald/GERALD.pl (/data3/Solexa2/Vasatzis/111408_30J7KAAXX_300164_20/Data/config.txt) returned error code 2
    Error: Failed to process Gerald configuration.
    --
    bioinfosm

  • #2
    Try just:
    ANALYSIS sequence_pair
    SEQUENCE_FORMAT --fastq
    nothing else.

    Comment


    • #3
      There should be a file called PairedEndInfo.xml in the run folder that the pipeline looks for to know the sequences length. Is this file present and you are using GAPipeline 1.0?

      Comment


      • #4
        PairedEndInfo.xml it was!

        Although mine read like this-
        $ cat ../../PairedEndInfo.xml
        <?xml version="1.0"?>
        <FirstRead Length="36" />

        I added the line
        <SecondRead Length="36" />
        --
        bioinfosm

        Comment


        • #5
          ___________________________
          Last edited by bioinfosm; 02-02-2009, 12:50 PM. Reason: <silly me error>
          --
          bioinfosm

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