Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • jmartin
    Member
    • Dec 2009
    • 78

    #1

    Need simple way to get coverage per 10kb interval on reference

    Is there a tool that can tell me coverage per some uniform interval along a set of reference contigs? I know I could use bedtools coverage and build a bed file defining the intervals, but I was asked if there wasn't already a tool that could just do uniform intervals on its own without having to setup a bedfile to define the intervals.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Use mosdepth and option --by 10000.

    You can also use deepTools and option multiBamSummary bins.

    Comment

    • Mizzou55
      Junior Member
      • Mar 2010
      • 7

      #3
      Does the --by option sum up the coverage for an interval (10000), or does it provide the depth at each interval?

      Comment

      • jmartin
        Member
        • Dec 2009
        • 78

        #4
        Thanks for the suggestion, mosdepth is just what I need!

        @Mizzou55 it looks like its reporting the average depth across the requested intervals. If you want depths at specific positions in your reference I guess you'd have to setup a bed file with the positions you want reported

        Comment

        Latest Articles

        Collapse

        • kinpict.com
          Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by kinpict.com
          The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
          Today, 06:37 PM
        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 11-12-2026, 07:59 AM
        0 responses
        27 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        2 responses
        56 views
        0 reactions
        Last Post kinpict.com  
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        175 views
        0 reactions
        Last Post SEQadmin2  
        Working...