Is there a tool that can tell me coverage per some uniform interval along a set of reference contigs? I know I could use bedtools coverage and build a bed file defining the intervals, but I was asked if there wasn't already a tool that could just do uniform intervals on its own without having to setup a bedfile to define the intervals.
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Does the --by option sum up the coverage for an interval (10000), or does it provide the depth at each interval?Comment
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Thanks for the suggestion, mosdepth is just what I need!
@Mizzou55 it looks like its reporting the average depth across the requested intervals. If you want depths at specific positions in your reference I guess you'd have to setup a bed file with the positions you want reportedComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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