Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • teaelleceecee
    Junior Member
    • Apr 2015
    • 1

    Program to view multiple alignment files at once

    I have aligned reads to a database containing 10 reference sequences using BWA MEM, and now have .sam and .bam files.

    Is there a program that would allow me to few those 10 alignments within the one .sam/.bam file all at once?

    I am currently using Tablet, but this only allows me to view the alignment for each reference sequence one at a time.

    Alternatively, is there a program that would allow me to view multiple .sam/.bam files at once, when each of those .sam/.bam files have only 1 reference sequence?

    I'm sorry if this question has been answered before but I couldn't find a post that answered this for me.

    Thank you for your help.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Are you sure about tablet not being able to open more than one alignment?

    In any case IGV will allow you to open multiple files (aligned to same genome). You will need to make sure you have enough RAM available and change the startup script for IGV to make more RAM available as needed.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    49 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    108 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    125 views
    0 reactions
    Last Post SEQadmin2  
    Working...