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  • frankenndoc
    Junior Member
    • Mar 2019
    • 7

    #1

    Parallelising Hisat2

    I need to run Hisat2 on 36 paired-end files. The files are labelled like:
    file1_1.fq
    file1_2.fq
    file2_1.fq
    file2_2.fq

    For unpaired reads I would use GNUParallel with hisat2 like:
    ls *.fq | parallel "hisat2 --dta -x ~/Databases/grch38_tran/genome_tran -U {} -S {.}sam" &

    But since for the pairs Hisat2 expects the two files to be input separately (-1, -2) options I don't know how to proceed. Any ideas?
    Last edited by frankenndoc; 03-07-2019, 12:33 PM. Reason: typo

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