I did some searching and came up with very little about how to effectively demultiplex illumina data off the GAiix platform. Does anyone know of a good software package or workflow I could read through and experiment with? Sorry if this question has already been asked, I wasn't able to find much information though
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Are you using your own barcodes or the Illumina multiplexing kit? The most recent version of NextGENe handles the demultiplexing of the data from the kits. I'm also trying to get it to work with CLC Genomics Workbench, but so far they aren't sorting right. Both of these programs cost $$ and I'm unfortunately not aware of free software that does it, although I'm sure it exists and someone else might know. Illumina software (CASAVA) will demultiplex data generated from their kits. It sorts into bins 1-24 (or 1-48?) but make sure to check what adapter/barcode is sorted into each bin because the numbers 1-24 don't correspond to adapters 1-24. I hope that helps!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
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