I want to mask primer sequences from analysis, but I want to keep the sequences in the FASTQ to help with alignment. Searching against a primer list and changing the quality scores to zero for matching bases would effectively prevent those bases from being included in analysis, and the preserved bases would aid in aligning the target sequence. Is there a method or tool to do this?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM