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  • ndovro
    Junior Member
    • Oct 2019
    • 1

    Discrepancy between samtools IDXSTATS and depth

    Hi all,
    i am baffled from some results and i was wondering if anyone can help.

    I am doing RNAseq.

    I got my sample, aligned it with STAR on a known reference and used samtools idxstats on the produced sortedbycoord file after indexing.

    I get all my contigs/chroms and then run samtools depth for each one of them with -aa.

    For some, even though idxstats reports a handful of mapped reads, I get 0 for all the bps with depth.

    What am i missing?

    Thanks for your time

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