RNASeq, Whole Genome & MetaGenomics Training in Dubai

[BDGLifescience]

2-day Technical Training in NGS Data Analysis | DNA, RNA & MetaGenomics in Dubai
When: 6-7 April 2020
Where: Executive Lounge, Business Centre, Level 30, The H Hotel-Office Tower, #1 Sheikh Zayed Road, Dubai, United Arab Emirates
This will be our 6th event in Dubai

Event Webpage

PRACTICAL APPLICATION: In the workshop, we will perform 3 experiments to analyze the example RNASeq, DNASeq & MetaGenomics data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.
SCHEDULE | DAY 1
EXPERIMENT 1
In this lesson, we will focus on the SNPs and Variant Calling (VC) of the DNASeq data and cover:

• Deal with the raw data for quality evaluation and filtering
• Map reads to the reference genomes, call SNPs and predict their phenotype consequence
• Alignment to a reference genome, SNPs and In-dels Calling of next-generation sequencing DNA data

SCHEDULE | DAY 2
EXPERIMENT 2
In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:

• Upload Data
• Convert File Format
• Align Reads To Reference Genome
• View The Mapped Reads In JBrowse
• Count Reads Per Gene
• Test For Differential Expression
• Differential Gene Expression
• MDS Plot
• MA Plot
• Volcano Plot
• Parallel Coordinates and Heatmap
• Investigate Differentially-Expressed Genes
• Chord Diagram

EXPERIMENT 3
In this lesson, we will focus on the MetaGenomics Analysis in 16S rRNA | Amplicon Sequencing Data Analysis (OTUs) and will cover:

• File Optimization- Loading of data & optimizing it according to our experiment.
• Quality Control- Check sequencing quality
• Sequence Alignment- Grouping/Alignment of reads
• Extraction of Taxonomic Information- Operational Taxonomic Units (OTUs)- Clustering Sequences
• Visualization- Examine diversity of species/taxa present within a single sample, Comparison of species/taxa diversity between two samples & Taxonomic Diversity- How many species are in a sample? What are the differences in species between samples?