I am trying to do a de novo assembly using Trinity. Using Transdecoder and blastp/hmmscan search to do annotation (using Uniprot/pfam databases). https://github.com/TransDecoder/TransDecoder/wiki . But i would like to do a blastp/hmmscan search only for a specific taxonomy, viridiplantae to be more precise (taxid: 33090). I managed to find a Uniprot database for plants only, but my problem is how to find a pfam database specific for plants. Here : http://pfam.xfam.org/search#tabview=tab1 there is the taxonomy tab, but when checking the box "find unique.." it returns an error. Thanks in advance
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM