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  • gen2prot
    Member
    • Apr 2010
    • 68
    #1

    Minimum transcriptome requirement for genome annotation

    Hi,

    I would like to know if there is any information available on the minimum number of transcriptomes required to get a genome annotation that predicts 90% of the genes. For example, If you are building a genome annotation (specifically for gene) for a new species, and would like to generate a reasonably comprehensive annotation in that species (90% of the genes and gene models captured), then how many transcriptomes would help you?

    I am thinking 3 tissues x 3 developmental stages, so 9 transcriptomes. Does anyone have a different suggestion on how to approach this? or a publication you can point me to?

    Thanks
    Abhijit
    Tags: annotation, rna-sequencing, transcriptome
  • luc
    Senior Member
    • Dec 2010
    • 469
    #2
    For a plant or an animal?

    Comment

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